A simple, fast, and orientation-controllable technology for preparing antibody-modified liposomes
نویسندگان
چکیده
Modification with antibodies is a useful strategy for the delivery of nanoparticles to target cells. However, complexity required chemical modifications makes them time-consuming and low efficiency, orientation antibody challenging control. To develop simple, fast, effective, orientation-controllable technology, we employed staphylococcal protein A, which can bind Fc region antibodies, as tool conjugating nanoparticles. Specifically, modified C-domain dimer A contain lysine cluster create molecule, DPACK, that would electrostatically anionic liposomes. Using this protein, antibody-modified liposomes be prepared in 35 min two steps: (1) interaction DPACK (2) an DPACK-modified Binding efficiencies IgG were 75% 72–84%, respectively. Uptake anti-epidermal growth factor receptor (EGFR) via by EGFR-expressing cancer cells was significantly higher than unmodified liposomes, accumulated tumors colocalized EGFR. This effective technology preparing will active targeting drug delivery.
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ژورنال
عنوان ژورنال: International Journal of Pharmaceutics
سال: 2021
ISSN: ['0378-5173', '1873-3476']
DOI: https://doi.org/10.1016/j.ijpharm.2021.120966